Linear chain PEGylated recombinant Bacillus thiaminolyticus
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Therapeutic Discovery Linear Chain PEGylated Recombinant Bacillus Thiaminolyticus Thiaminase I Enzyme Has Growth Inhibitory Activity against Lymphoid Leukemia Cell Lines
Cancer cells acquire abnormalities in energy metabolism, collectively known as the Warburg effect, affecting substrate availability of thiamine-dependent enzymes. To investigate a strategy to exploit abnormal cancer-associated metabolism related to thiamine, we tested the cytotoxicity of native Bacillus thiaminolyticus thiaminase I enzyme, which digests thiamine, in the NCI60 cell line drug cyt...
متن کاملLinear chain PEGylated recombinant Bacillus thiaminolyticus thiaminase I enzyme has growth inhibitory activity against lymphoid leukemia cell lines.
Cancer cells acquire abnormalities in energy metabolism, collectively known as the Warburg effect, affecting substrate availability of thiamine-dependent enzymes. To investigate a strategy to exploit abnormal cancer-associated metabolism related to thiamine, we tested the cytotoxicity of native Bacillus thiaminolyticus thiaminase I enzyme, which digests thiamine, in the NCI60 cell line drug cyt...
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The second thiamine-decomposing bacterium, discovered by Kimura et al. (1, 2) was named by the Vitamin B Research Committee Bacillus aneurino lyticus Kimura et Aoyama (BKA) (3). By studying the properties of the enzyme produced by this bacillus, it was found that the action mechanism of the enzyme is completely different from that of Bacillus thiaminolyticus Matsukawa et Misawa (BMM), shell-fis...
متن کاملPharmacologic properties of polyethylene glycol-modified Bacillus thiaminolyticus thiaminase I enzyme.
We have previously shown that the bacterial enzyme thiaminase 1 has antitumor activity. In an attempt to make thiaminase I a more effective pharmaceutical agent, we have modified it by adding polyethylene glycol (PEG) chains of various lengths. We were surprised to find that 5k-PEGylation eliminated thiaminase cytotoxic activity in all cell lines tested. Both native thiaminase and 5k-PEGylated ...
متن کاملSite-Specific PEGylation of Recombinant Immunotoxin DAB389IL-2: Structural and Functional Assessment
Background: DAB389IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB389IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB389IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is co...
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